Scattering microscopy of single particles

The ILM has developed an angularly and spectrally resolved light scattering microscope for the characterisation of biological and technical single scatterers such as cells or colour pigments. In the first mode, two-dimensional angularly resolved measurements can be

performed at discrete tunable wavelengths. The second mode allows spectrally resolved measurements between 450 nm and 950 nm integrated over the entire detected angular range, see sketch.

Possible applications of the light scattering microscope

  • Determination of particle sizes of scatterers of known geometry to an accuracy of a few nanometers
  • Determination of the spectrally resolved refractive index of particles of known geometry
  • Characterization of bacteria (e.g. E. coli) or cells
  • Characterization and categorization of chromosomes
Applications of the scattered light microscope

Contactpersons

Prof. Dr. Alwin Kienle

Prof. Dr. Alwin Kienle

Director Materials Optics & Imaging

Tel: +49 (0)731 / 1429 224

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